Table 8 Analysis of variance (ANOVA) of BBD of AuNPs biosynthesis, using the extracellular filtrate of Bacillus subtilis NRC1.

From: Optimization of Bacillus subtilis NRC1 growth conditions using response surface methodology for sustainable biosynthesis of gold nanoparticles

Source

Sum of squares

df

Mean square

F-value

p-value* prob > F

Model

26.42

23

1.15

14.40

 < 0.0001

A-casein hydrolysate

3.66

1

3.66

45.87

 < 0.0001

B-dextrin

0.01

1

0.01

0.18

0.6750

C-intial pH

4.47

1

4.47

56.09

 < 0.0001

D-inoculum size

0.13

1

0.13

1.69

0.2077

E-temperature

0.34

1

0.34

4.22

0.0520

AB

0.38

1

0.38

4.82

0.0390

AC

0.59

1

0.59

7.43

0.0123

AD

1.15

1

1.14

14.36

0.0010

AE

0.80

1

0.80

10.04

0.0044

BC

1.58

1

1.58

19.75

0.0002

BD

1E−04

1

1E−04

0.00

0.9721

BE

0.09

1

0.09

1.21

0.2842

A2

0.06

1

0.06

0.71

0.4098

B2

0.01

1

0.01

0.11

0.7424

C2

0.46

1

0.46

5.73

0.0256

D2

0.09

1

0.09

1.12

0.3017

A2B

2.33

1

2.33

29.25

 < 0.0001

A2D

1.54

1

1.54

19.28

0.0002

AB2

1.63

1

1.63

20.40

0.0002

AD2

2.70

1

2.70

33.86

 < 0.0001

B2E

0.67

1

0.67

8.39

0.0083

BC2

0.06

1

0.06

0.79

0.3837

BD2

2.08

1

2.08

26.09

 < 0.0001

Residual

1.75

22

0.08

  

Lack of fit

1.53

17

0.09

2.02

0.2241

Pure error

0.22

5

0.05

  

Cor total

28.17

45

   
  1. F value = 14.40; R2 = 0.94; Adjusted R2 = 0.87.
  2. *: Values of "prob > F" less than 0.05 indicate model terms are significant.