Table 7 Primers’ sequences and PCR conditions.
Gene | SNP | Primers (5′–3′) | PCR conditions |
|---|---|---|---|
TGFB1 | C-509T | F:GTCGCAGGGTGTTGAGTGACAG R:AGGGGGCAACAGGACACCTTA | 95 °C, 5 min- initial denaturation, 95 °C, 30 s; 62 °C, 45 s; 72 °C, 1 min- 35 cycles, 72 °C, 10 min- final elongation |
TGFB1 | Leu10Pro | F:CTCCGGGCTGCGGCTGCAGC R:GGCCTCGATGCGCTTCCGCTTCA | 95 °C, 5 min- initial denaturation, 95 °C, 30 s; 62 °C, 45 s; 72 °C, 1 min- 35 cycles, 72 °C, 10 min- final elongation |
XRCC1 | Arg280His | F:TGGGGCCTGGATTGCTGGGTCTG R:CAGCACCACTACCACACCCTGAAGG | 94 °C, 5 min- initial denaturation, 94 °C, 40 s; 55 °C to 69 °C, 40 s; 72 °C, 30 s- 35 cycles, 72 °C, 10 min- final elongation |
XRCC3 | Thr241Met | F: GGTCGAGTGACAGTCCAAAC R: TGCAACGGCTGAGGGTCTT | 95 °C, 5 min- initial denaturation, 95 °C, 1 min; 60 °C, 1 min; 72 °C, 1 min- 40 cycles, 72 °C, 5 min- final elongation |