Table 7 Primers’ sequences and PCR conditions.

From: Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer

Gene

SNP

Primers (5′–3′)

PCR conditions

TGFB1

C-509T

F:GTCGCAGGGTGTTGAGTGACAG

R:AGGGGGCAACAGGACACCTTA

95 °C, 5 min- initial denaturation,

95 °C, 30 s; 62 °C, 45 s; 72 °C, 1 min- 35 cycles,

72 °C, 10 min- final elongation

TGFB1

Leu10Pro

F:CTCCGGGCTGCGGCTGCAGC

R:GGCCTCGATGCGCTTCCGCTTCA

95 °C, 5 min- initial denaturation,

95 °C, 30 s; 62 °C, 45 s; 72 °C, 1 min- 35 cycles,

72 °C, 10 min- final elongation

XRCC1

Arg280His

F:TGGGGCCTGGATTGCTGGGTCTG

R:CAGCACCACTACCACACCCTGAAGG

94 °C, 5 min- initial denaturation,

94 °C, 40 s; 55 °C to 69 °C, 40 s; 72 °C, 30 s- 35 cycles,

72 °C, 10 min- final elongation

XRCC3

Thr241Met

F: GGTCGAGTGACAGTCCAAAC

R: TGCAACGGCTGAGGGTCTT

95 °C, 5 min- initial denaturation,

95 °C, 1 min; 60 °C, 1 min; 72 °C, 1 min- 40 cycles,

72 °C, 5 min- final elongation