Figure 6 | Scientific Reports

Figure 6

From: Caffeic acid N-[3,5-bis(trifluoromethyl)phenyl] amide as a non-steroidal inhibitor for steroid 5α-reductase type 1 using a human keratinocyte cell-based assay and molecular dynamics

Figure 6

Expression levels of SRD5A1 mRNA and protein after treatment with various concentration of compound 4 (0 − 2.5 µM) as relative of the untreated control cells at 12 h and 24 h. (a) Expression level of SRD5A1 mRNA at 12 h and 24 h. The result mRNA expression was represented as Target gene/GAPDH and was analyzed via qRT-PCR. (b) Expression level of SRD5A1 protein at 12 h. (c) Expression level of SRD5A1 protein at 24 h. (d) SRD5A1 enzyme activity at 12 h (e) SRD5A1 enzyme activity at 24 h. All data were obtained in triplicate experiments and expressed as the mean ± SEM. **p < 0.001, ***p < 0.0005, ****p < 0.0001 versus untreated control cells (Ctrl).

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