Table 2 List of PCR primer used in this work.
From: The ground beetle Pseudoophonus rufipes gut microbiome is influenced by the farm management system
Primer Name | Sequence (5′-3′) | Amplicon size | Reference | |
|---|---|---|---|---|
Eubacteria | Eub338-F | ACTCCTACGGGAGGCAGCAG | 200 | Muyzer et al. (1993) |
Eub518-R | ATTACCGCGGCTGCTGG | |||
Illumina adapter—V3-V4 Region of 16S rRNA gene | Pro341-F | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCCTACGGGAGGCAGCAG-CCTACGGGNGCASCAG | 560 | Takahashi et al. (2014) |
Pro805-R | CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-GACTACNVGGGTATCTAATCC |
