Figure 2
From: Effects of small molecule-induced dimerization on the programmed death ligand 1 protein life cycle
Overall turnover of PD-L1 protein. (A) Degradation of PD-L1 3xFLAG protein transfected into HEK 293 T cells measured by SILAC pulse chase method. Data for DMSO control are superimposed with those for dimer-inducing 1 μM GS-4224 and Incy-11, and 1 μM macrocyclic blocker BMS-10034. The half-life comparisons demonstrate that DMSO and BMS-10034 have half-life estimates that are not statistically different (p > 0.10) yet these are statistically greater (p < 0.0001) than the GS-4224 and Incy-11 half-life estimates. Also, GS-4224 has a half-life estimate that is statistically less (p < 0.001) than that of Incy-11. (B) Total endogenous PD-L1 levels in MDA-MB-321 GFP cells measured over 48 h by WB. GS-4224 treated samples are compared to DMSO control and BMS-10034 treated cells. Actin is used as a loading control.
