Figure 4

Microscopy analysis of PD-L1 cellular localization. (A) CHO cells overexpressing PD-L1, which is detected with anti-PD-L1 antibody (red). Intracellular space and nuclei stained with Calcein AM (green) and Hoechst (blue), respectively. The detection antibody was applied after 1-h of compound treatment. (B) MDA-MB-231 cells treated with GS-4224 for 24 h, fixed and stained with anti-PD-L1 antibody (red). Cell surface mask (white) was created using an anti-Na⁺/K⁺-ATPase antibody staining (green). PD-L1-specific signal at cell surface is plotted against concentration of dimerizing compound GS-4224.