Figure 7
From: Effects of small molecule-induced dimerization on the programmed death ligand 1 protein life cycle
Intracellular accumulation of PD-L1. (A) Expi293 cells overexpressing FLAG-PD-L1, which is detected with anti-FLAG antibody (green). Nuclei are stained with Hoechst (blue). (B) Expi293 cells treated with GS-4224 for 24Â h, fixed and stained with anti-FLAG antibody (green) to detect PD-L1; anti-calnexin antibody (red) to label ER, and Hoechst (blue) to visualize nuclei.
