Figure 3 | Scientific Reports

Figure 3

From: Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway

Figure 3

Oxidation of G6PDH and 6PGDH mediated by AAPH-derived ROO• affects the molecular mass of the proteins and generates aggregates and fragments. Solutions of G6PDH (54 μM monomer) or 6PGDH (58 μM monomer) were incubated in the absence and presence of 100 mM AAPH at 37ºC in 75 mM phosphate buffer, pH 7.4. Aliquots of these solutions were taken every 30 min up to 180 min, AAPH removed as described in the Materials and methods, and samples kept at -80ºC until subjected to electrophoresis analysis. Panel A and B present SDS-PAGE of G6PDH and 6PGDH, respectively. SDS–PAGE was run under reducing conditions on 4–12% Bis–Tris acrylamide gels with 60 μg of protein loaded per well. MM: molecular mass markers, with masses indicated on left hand vertical axis. Lane C shows the electrophoretic pattern of proteins incubated for 180 min in the absence of AAPH.

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