Figure 6 | Scientific Reports

Figure 6

From: Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway

Figure 6

Quantification of the parent amino acids Trp, Tyr, His and Met as detected by LC–MS in G6PDH (54 μM monomer, Panel A) and 6PGDH (Panel B, 58 μM monomer) after incubation in the absence, or presence, of 10 and 100 mM AAPH at 37 °C for 180 min. The vertical axis indicates the % modification for each residue in the different conditions for control (red), 10 mM AAPH (blue) and 100 mM AAPH (orange) samples. The data are mean values obtained from the analysis of three different replicates prepared on different days. The % modification was calculated considering the full sequence of each protein G6PDH (P0AC53) and 6PGDH (P00350). The data corresponding to the quantification of the % modification of individual residues in the sequence of both proteins, with their respective standard deviations, is available in the Supplementary data.

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