Table 1 AAPH-derived ROO· affect the amino acid content of G6PDH and 6PGDH.

From: Implications of differential peroxyl radical-induced inactivation of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase for the pentose phosphate pathway

AAPH (mM)

Trp

Met

10

100

10

100

G6PDH

Consumption (μM)

80.0 (15.0%)

140.1 (26.0%)

20.0 (17.0%)

102.4 (18.9%)

Per ROO·

0.56

0.09

0.14

0.07

Per protein

1.5

2.6

0.37

1.9

6PGDH

Consumption (μM)

29.0 (4.0%)

63.8 (36.6%)

22.0 (7.0%)

121.8 (23.9%)

Per ROO·

0.2

0.044

0.15

0.09

Per protein

0.5

1.1

0.38

2.4

  1. Consumption of amino acids was assessed by OPA assay after incubation of G6PDH (54 μM) or 6PGDH (58 μM) with 10 and 100 mM AAPH for 180 min at 37 °C. Data are expressed as μM concentrations. The ratios of amino acid consumption (μM) and the total dose of ROO· generated into the system (0.144 or 1.44 mM for 10 or 100 mM AAPH), and protein concentration (μM), are presented. Such ratios give values meaning the moles of amino acid consumption per mole of ROO·, and per mole of protein, respectively. Values in parenthesis show the % consumption of each amino acid, taking the initial values as 100%. Data are the mean of at least three independent experiments each measured in triplicate. For ease of reading only the standard deviations of loss of enzymatic activity are shown.
Back to article page