Figure 2
Effect of UVA irradiation on fibroblast cells. MTT cell viability assay was employed to assess the percentage of viable cells. Un-irradiated control (0 kJ/cm2) represent 100% cell viability (A). Detection of NO in UVA-irradiated fibroblast cells and effect of the EC50 concentration of compounds on the production of NO. The amount of NO (µM) was measured by Griess reagent assay following the manufacturer’s instructions. The bar graph represents the levels of NO generated (%) and the scatter plot shows the NO scavenging activity (%) of the compounds in presence of UVA (B). Detection of ROS in UVA exposed dermal fibroblast cells and the effect of an EC50 concentration of compounds on ROS generation and ROS scavenging activity. The production of ROS was measured by using H2DCFDA fluorescent probe. The bar graph showed the generation of ROS (%) and the scatter plot exhibited intracellular ROS scavenging activity (%) (C). All values are represented as mean ± S.E.M (n = 3).
