Figure 4
Treatment cycle. Red: PDI with 10 μM of curcumin and 10 J/cm2 and Blue: antibiotic group. Normalizations performed for untreated bacteria. (A) Combined treatment switched every 6 h, starting with PDI (switch.ANTB). (B) Combined treatment—concomitant PDI and ANTB every 6 h (PDI/ANTB). (C) FTIR absorbance spectrum of surviving bacteria after 12 h of treatment cycle of PDI, AMO, ERY, and GEN monotherapies; sequentially combined treatments every 6 h (PDI/ANTB), and switching PDI and antibiotics every 6 h (switch.ANTB). Hierarchical Cluster Analysis of the 2nd derivative of the FTIR absorption spectrum of regions (D) 900–1200 cm−1, corresponding to carbohydrates, (E) 1500–1800 cm−1, corresponding to protein, and (F) 2800–3100 cm−1, corresponding to fatty acids. Colors of the dendrogram indicate clustering of similarity greater than 50%. A statistically significant difference was considered p < 0.05.
