Figure 7
Evaluation of spontaneous AP and CaT characteristic measured by patch clamp technique in perforated cells. Panel a indicates that in the presence of 1 µM strophantin (green curve) both the AP cycle length (upper part) and the Ca2+-transient amplitude (lower part) was increased compared to control (black curve). Bar graphs illustrate statistically significant change of the cycle length and diastolic slope in the presence of strophantin (panel b). In contrast, when the cells were pre-treated with 1 µM ORM-10962 (panel c, pink curves) both the action potential cycle length (upper part) and the Ca2+ transient amplitude (lower part) remained unchanged. As bar graphs show none of the investigated parameters changed when the NCX was inhibited prior to strophantin employment (panel d). Data shown as mean ± SEM, n = 6, paired t-test, *means p < 0.05.
