Figure 3 | Scientific Reports

Figure 3

From: The mitochondrial Ca2+ uniporter channel synergizes with fluid shear stress to induce mitochondrial Ca2+ oscillations

Figure 3The alternative text for this image may have been generated using AI.

MCU OX causes mROS flashes and ΔΨm flickers in sheared ECs. (A) Characteristic temporal profiles of normalized mtSOX Deep Red fluorescence for each EC in a microscope image, where the monolayer was exposed to 1 min static followed by 9 min SS. (B) Same as in (A), but for Ad.MCU-transduced ECs. (C) Magnification of a ΔΨm transient (shown by a dotted box in B). (D) Characteristic TMRM fluorescence shows overlap with mito-GCaMP6 in an unstimulated EC. (E) F (in arbitrary units) averaged over an area of a cell (white box in D) for both mito-GCaMP6 and TMRM vs. time. (F) Magnification of the cell area (white box in D) shows opposing behavior between mito-GCaMP6 and TMRM at a single-mitochondrion level at time points 1–3 (time points shown in E).

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