Figure 4
From: AS-CMC: a pan-cancer database of alternative splicing for molecular classification of cancer
“Pan-cancer comparison” using AS-CMC. (a) Selection panel for AS events. Users are directed to the pan-cancer views upon clicking on an AS event. (b–e) Pan-cancer information pertaining to MAP3K7 exon 11 as an example. (b) Comparison of subtype-specificity across cancer types. ANOVA results are displayed using two different y-axes: − log10 (p-value) and adjusted R2 values on the left (green) and right (blue), respectively. The two cut-offs to select subtype-specific AS events are marked by two dotted lines: − log10 (p-value) > 3 and adjusted R2 > 0.1. The significant cancer types are marked by red on the x-axis. (c) Pan-cancer comparison of hazard ratios and log rank p-values obtained in survival analysis. The dot size indicates p-values from log rank test. Each cancer type has three dots, which match with three survival tests based on PSI cut-offs. The darkest red color indicates the comparison between two patient groups (group with PSI values higher than 10% and one with that lower than 10%). The medium red color indicates 25% cut-off and the lightest red color indicates 50% cut-off used for generating two groups. (d) Heatmap showing correlation coefficients with the gene harboring the AS event. The color key on the top shows the distribution of correlation coefficients across cancer types. (e) Heatmap showing correlation pattern for a given AS event with gene expression levels. Each cell contains Pearson’s correlation coefficients (r) between MAP3K7 AS PSI values and expression level of each gene for a given cancer type. Only those genes showing |r|> 0.5 in any cancer type are included. The cancer types and genes are arranged by hierarchical clustering (Euclidean distance and complete linkage method). EMT-related genes are shown on the right, and the cancer types with similar correlation pattern are marked by a red dotted box, on this plot in AS-CMC.
