Table 3 Recommended range of optimal disruption times using adult rat ILMs.

From: An optimized method for high-quality RNA extraction from distinctive intrinsic laryngeal muscles in the rat model

Muscle type

PCA

LTA

MTA

Mass (mg)a

Time (s)

Mass (mg)a

Time (s)

Mass (mg)a

Time (s)

    

10 mg (n = 5)

25–30 s

  

20 mg (n = 2)

35 s

20 mg (n = 9)

30 s

  

30 mg (n = 4)

35 s

30 mg (n = 4)

30–35 s

40 mg (n = 9)

65–70 s

40 mg (n = 11)

35–40 s

40 mg (n = 2)

30–35 s

50 mg (n = 6)

70–75 s

    

60 mg (n = 3)

75 s

    
  1. The appropriate span of rotor–stator disruption attributed to the rodent stage, the total weight of the specimen, and muscle type are shown. The listed ranges were collated based on extraction efficiency.
  2. aTotal muscle weights using 3 pooled tissues.
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