Table 4 Summary of the downstream diagnostics from experimental total RNA extracts (N = 90).

From: An optimized method for high-quality RNA extraction from distinctive intrinsic laryngeal muscles in the rat model

Muscle

N

260/230

260/280

Total RNA (ng)

RIN

28S/18S

No. reads

Pseudo aligned reads

Mapping ratio

PCA

29

1.03 ± 0.53

2.15 ± 0.09

1513.68 ± 860.68

7.8 ± 0.3

1.4 ± 0.2

4.19E+07 ± 7.23E+06

3.33E+07 ± 5.88E+06

79.4 ± 1.5

LTA

30

1.08 ± 0.47

2.12 ± 0.06

1436.82 ± 903.31

7.9 ± 0.4

1.4 ± 0.2

4.22E+07 ± 7.42E+06

3.31E+07 ± 5.83E+06

78.5 ± 1.8

MTA

29

1.10 ± 0.45

2.13 ± 0.06

1081.44 ± 397.08

8.1 ± 0.5

1.4 ± 0.2

4.04E+07 ± 6.82E+06

3.13E+07 ± 5.45E+06

77.5 ± 1.7

Control

2

0.85 ± 0.36

2.14 ± 0.19

413.55 ± 25.63

8.1 ± 0.7

1.6 ± 0.1

4.17E+07 ± 7.22E+06

3.37E+07 ± 5.99E+06

80.9 ± 0.3

  1. RNA samples were sequenced using a standard RNA-Seq pipeline (Illumina NovaSeq 6000), and sequencing reads were quantified using Kallisto.
  2. Note, the controls in this experiment were (1) RNA extracts from the right ILMs aggregated and (2) RNA extracts from all paired ILMs combined.
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