Figure 1 | Scientific Reports

Figure 1

From: Distribution and postnatal development of chondroitin sulfate proteoglycans in the perineuronal nets of cholinergic motoneurons innervating extraocular muscles

Figure 1

Pattern of chondroitin sulfate proteoglycans around acetyl-cholinergic motoneurons (green) in the oculomotor nucleus. The nuclei were detected with DAPI. PNNs (red) were immuno-positive with WFA and aggrecan labeling (white arrows) (A,B). PNNs were absent around only the minority of cholinergic motoneurons labelled with WFA and aggrecan (A,B), and were entirely absent with brevican, neurocan and versican immuno-staining (asterisks) (C–E). Nevertheless, rod-like brevican accumulations gathered in the proximity of some motoneurons (C), although PNNs did not assemble. Somata in (C) appear in yellow tone due to the co-labelling of the cytoplasmic brevican and acetylcholine. In (E) dot-like accumulations of versican characterized the neuropil (yellow arrowheads). (F) Position of oculomotor- (nu3) and Edinger-Westphal (EW) nuclei in the mesencephalon (modified after Paxinos and Franklin35) Aq: cerebral aqueduct. MLF: medial longitudinal fasciculus. PAG: periaqueductal gray matter. D: dorsal. V: ventral. L: lateral. Scalebar: 10 µm.

Back to article page