Table 1 Lectin and primary antibodies used.
| Â | bWFAa | Anti-aggrecan | Anti-brevican | Anti-neurocan | Anti-versican | Anti-choline-acetyltransferase |
|---|---|---|---|---|---|---|
Supplier, cat. no. | Sigma-Aldrich; L1516 | Merck Millipore; AB1031 | R&D Systems; AF4009 | R&D Systems; AF5800 | Biorbyt; orb259630 | Merck Millipore; AB144P |
Species of origin, type | Lectin isolated from Wisteria floribunda; biotinylated | Rabbit; polyclonal, IgG | Sheep; polyclonal, IgG | Sheep; polyclonal, IgG | Rabbit; polyclonal, IgG | Goat; polyclonal, IgG |
Immunogen | - | GST fusion protein containing amino acids 1177–1326 of mouse aggrecan | Mouse myeloma cell line NS0 derived recombinant human brevican Asp23-Pro911 | Chinese hamster ovary cell line CHO derived recombinant mouse Neurocan Asp23-Asp637 | A synthetic peptide corresponding to a sequence at the N-terminus of human Versican | Human placental enzyme |
Dilution | 1:500 | 1:500 | 1:50 | 1:300 | 1:200 | 1:100 |
