Figure 1 | Scientific Reports

Figure 1

From: Direct thrombin inhibitors as alternatives to heparin to preserve lung growth and function in a murine model of compensatory lung growth

Figure 1

Lung endothelial cell proliferation and apoptosis. Bivalirudin (A) and argatroban (B) did not affect human lung microvascular endothelial cell (HMVEC-L)-L proliferation at various dosages. Heparin, however, inhibited proliferation of HMVEC-L cells in a dose-dependent manner (C). Bivalirudin consistently inhibited apoptosis (D). Argatroban increased apoptosis at a single dose (25 μg/mL) while remaining doses of argatroban did not impact apoptosis (E). Increasing doses of heparin increased HMVEC-L apoptosis (F). Each anticoagulant dose was performed in technical quadruplicate and the experiments were repeated up to 3 times. Comparisons were done using a one-way analysis of variance (ANOVA) with Dunnett’s adjustment for multiple comparisons. Shown are mean ± standard error. *P < 0.05; **P < 0.01; ***P < 0.001.

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