Figure 3 | Scientific Reports

Figure 3

From: Mesenchymal stromal cells donate mitochondria to articular chondrocytes exposed to mitochondrial, environmental, and mechanical stress

Figure 3

MSC-chondrocyte MT transfer is affected by non-physiologic culture conditions. Murine mCherry chondrocytes (red) and PhAM mitoDendra2 MSCs (green MT) were co-cultured in hyperoxia or hyperglycemia for 12 h after stimulation with or without Rot/A or IL-1β. MT transfer events (% red+/green+ cells) were quantified by flow cytometry. Hyperoxia increases transfer events in non-stimulated and IL-1β-stimulated chondrocytes (P < 0.05). Hyperoxia and hyperglycemia decreased transfer events when chondrocytes were stimulated with Rot/A (P < 0.0007). n = 4.

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