Figure 1 | Scientific Reports

Figure 1

From: Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnostics

Figure 1

Analysis of the experiment results aiming to minimize the Time To Reaction (TTR) response of positive reactions, obtained with the software Minitab. In (A) Residual plots. The normal probability plot represents that the residuals analyzed are normally distributed. The histogram represents the distribution of the residuals. The residual versus fits plot shows that the residuals are randomly distributed, and the residual versus order plot indicates the residuals in the order that the data were collected, showing that the residuals are independent since there are no patterns in the observation order. (B) Normal probability plot with α = 0.05, where the significant factors, in order, were: primer sequence (F), Bst concentration (B), primer concentration (A), magnesium sulfate concentration (C), and the combination of primer sequence and adjust of hydrochloride guanidine pH (FG).

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