Figure 5 | Scientific Reports

Figure 5

From: Pseudomonas aeruginosa aggregation and Psl expression in sputum is associated with antibiotic eradication failure in children with cystic fibrosis

Figure 5

Differences in tobramycin response and aggregation between persistent and eradicated P. aeruginosa isolates in glass slide chamber. Eradicated (n = 7) and persistent (n = 4) P. aeruginosa isolates grown for 24 h as biofilms in glass slide chamber and then treated with 1000 µg/mL tobramycin for an additional 24 h then visualized with confocal microscopy. LB: Lysogeny Broth alone; + T: plus Tobramycin; SS: in presence of 5% pooled CF Sputum Supernatant. Horizontal line, median. (A) average P. aeruginosa biovolume intensity (µm3)/well, (B) average luminescence in relative light units (RLU) of ATP assay/well, (C) average degree of aggregation measured by surface to biovolume ratio/well. *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant using Mann–Whitney test.

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