Table 1 Detail of primers used for identification of fungal pathogens in the present study.

From: Isolation and identification of Pithomyces sacchari as a leaf spot pathogen of Helianthus annuus from Pakistan

Sr. no.

Gene

Primer name

Sequence (5'–3')

References

1

Internal Transcribed Spacer Region

ITS1 (Forward)

ITS4 (Reverse)

5ʹ-TCC GTA GGT GAA CCT GCG G-3ʹ

5ʹ-TCC TCC GCT TAT TGA TAT GC-3ʹ

13

2

Glyceraldehyde-3-phosphate dehydrogenase

GAPDH (Forward)

GAPDH (Reverse)

5ʹ-CAA CGG CTT CGG TGG CAT TG-3ʹ

5ʹ-GCC AAG CAG TTG GTT GTG C-3ʹ

15