Figure 4 | Scientific Reports

Figure 4

From: Nanofiber formation as a promising technology for preservation and easy storage of extracellular vesicles

Figure 4The alternative text for this image may have been generated using AI.

Stability investigation of free mEV and mEV-loaded PVP fibers (PVP-mEVs) by flow cytometry. The stability of free mEVs and PVP-mEVs kept at 4 °C or RT was monitored for 12 weeks, every two weeks by flow cytometry. The stability was investigated based on the membrane-bound GFP signal of mEVs (A), the Violet-SSC parameter correlating with the vesicle size (B), CD81 expression (C) and particle number (D). Schematic illustrations of the hypothesized mechanism of the changes observed for free mEV and PVP-mEV samples kept at 4 °C are summarized in panel (E). nmEV-GFP = 3, nViolet-SSC = 3, nCD81 = 3, nparticle number = 2 (Figure was prepared using GraphPad Prism 7.00 for Windows, GraphPad Software, San Diego, California USA, http://www.graphpad.com).

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