Figure 1
From: STAT3 suppression and β-cell ablation enhance α-to-β reprogramming mediated by Pdx1
Ectopic expression of Pdx1 induces STAT3 activation in α cells. (A) αTC1cells were infected with Pdx1-expressing adenovirus (Ad-Pdx1) or a control adenovirus expressing GFP(Ad-GFP). Protein levels of phosphorylated STAT3 (pSTAT3), total STAT3, and GAPDH were assessed by Western blotting. IL-6-treated cells were used as a positive control. (B) Quantification of the Western blot shown in (A). The expression levels of pSTAT3 were normalized to those of total STAT3. *p < 0.05 (n = 3 for each group) (C) Immunocytochemical staining for Pdx1 (white) and phospho-STAT3 (pSTAT3, red) was performed in αTC1 cells 72 h after infection with a control adenovirus (Ad-GFP) or an adenovirus expressing Pdx1(Ad-Pdx1). STAT3 was activated in αTC1 cells with high expression of PDX1 (arrows), whereas STAT3 was not activated in cells with weak expression of PDX1 (arrowhead). Scale bars, 20 μm. The original blots/gels are presented in Fig. S10.
