Table 1 Oligonucleotides used in this study.
Oligonucleotide | Oligonucleotide sequence (5′–3′) | Method |
|---|---|---|
5Sr-1a | GAGACTGCCTGGGAATACC | Primer for PCR amplification of rDNA-related sequence |
5Sr-1b | GTCTCCCATCAAGTACTAACC | |
TrA-2a | CAACATGATTGGTCCA | Primer for PCR amplification of genomic SSH A sequence |
TrA-2b | GGGTGATTTACCTCGA | |
TrB-1a | GGCTCATAGAAGGAAGAAGC | Primer for PCR amplification of genomic SSH B sequence |
TrB-1b | CAACATCGCCACCTACATG | |
EEEb6-1a | CTCAGTAAKAAACRTCG | Primer for PCR amplification of genomic SSH C sequence |
EEEb6-1b | GTTAAACCACGCCCAC | |
Nested PCR Primer 1 | TCGAGCGGCCGCCCGGGCAGGT | Primer for direct colony PCR |
Nested PCR Primer 2R | AGCGTGGTCGCGGCCGAGGT | |
M13-F | GTAAAACGACGGCCAGT | |
M13-R | CAGGAAACAGCTATGAC | |
EEEb3-f | AAATGCTGAGCATCGCG | Insert PCR for Southern-blot hybridization |
EEEb3-r | GGCCAGAGAACTTGTG | |
EEEb1-probe | GTTTCATGGTTATGGACCGTCGTTCCGCGTCGGCGTGTGA | Probe for slot-blot hybridization |
EEEb2-probe | AAAGTTTGAGTGGTGTAAGAAGTTATAATGTTTTTTTTGAGGTGAGATGTTTATTTT | |
EEEo1-probe | TATCTCAAAAAGTTGTKTAYGGNTTCGGAYGAAACTTGGTGGACACGTTGG | |
EEEo2-probe | TGGAAGTGATTTTTTCTCCTTTTTTGTGGTCAGAAGTGGTTATTTTTCATTGTTTTT | |
EEPa1-probe | AAAGTGAAAAGTGCCCCCTGGTGGATGATGGAAGGA | |
EEPs1-probe | CGGAACTCGTCGTGCGTTTTGCACATGCGCGAACGACCCC | |
EEEb4-probe | CCAGCGCGATCACGTGCCGCCGAGTAGAAGATGTGCACCT CGAGGTAAATCACCCAACATGATTGGT | |
EEEb5-probe | CTCATAGAAGGAAGAAGCATCACATGATGTTATATGGACATGTAGGTGGCGATGTTGG | |
EEEb6-probe | GGTGTGGGCGTGGTTTAACTCAGTAAGAAACGTCGAGAATGTAATTTTATAAATCG | |
EEEb3-f | AAATGCTGAGCATCGCG | Primer for recloning of EEEb3 |
EEEb3-1b | GTGCCATGTTTGGTGGTATAA |
