Figure 2 | Scientific Reports

Figure 2

From: Controlling anammox speciation and biofilm attachment strategy using N-biotransformation intermediates and organic carbon levels

Figure 2

Phylogenetic tree based on 16S rRNA sequences of major OTUs (with postfix “*”) and clones (with postfix “**”) from amplicon sequencing and clone library analysis, respectively. Number of identical colonies per total colonies picked is indicated in parentheses, e.g. 34/40 indicated 34 identical colonies per 40 colonies picked. The phylogenetic tree was generated by ARB with SILVA database. Sequences obtained from clone library and amplicon sequencing were inserted into the tree using parsimony insertion tool of ARB. The closest neighbor sequences were selected to generate the final tree with neighbor-joining method with bootstrap of 1000 replications. Dashed line indicates the division of family Ca. Brocadiaceae and genus Ca. Brocadia. Methanosaeta concilii was selected as the outgroup. Only the closest identified sequences were selected to be shown. The scale indicates 0.1 nucleotide change per nucleotide position. Sequence affiliations and relative abundances of major anammox bacteria were consistent between amplicon sequencing and clone library analysis.

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