Table 7 Experimental design and results of respiratory metabolic response surface.

From: The combined effects of temperature and salinity on the digestion and respiration metabolism of Pinctada fucata

Run

Coded

Actual

LDH

NKA

AKP

GPX-gill

SOD-gill

T (℃)

S (‰)

T (℃)

S (‰)

(AU/gprot)

(AU/mgprot)

(King unit /gprot)

1

0

1

25

33

317.87

2.26

151.31

0.901

0.6010

2

−1

0

20

28

341.31

3.12

319.61

0.840

1.1900

3

0

0

25

28

322.16

1.92

223.45

0.565

0.4219

4

−1

−1

20

23

556.99

4.61

314.79

1.110

2.2400

5

0

1

25

33

317.87

2.16

181.31

0.930

0.6100

6

0

0

25

28

300.68

2.02

223.45

0.385

0.7819

7

1

−1

30

23

515.69

0.92

454.22

0.426

0.3266

8

−1

0

20

28

361.31

2.92

299.61

0.870

1.2900

9

0

−1

25

23

435.70

2.77

316.74

0.661

0.6130

10

0

0

25

28

287.16

2.22

223.45

0.435

0.4919

11

1

0

30

28

442.79

1.07

324.91

0.391

0.6600

12

0

0

25

28

312.16

2.01

223.45

0.445

0.3119

13

1

1

30

33

505.90

1.18

248.22

0.726

1.0200

14

0

−1

25

23

435.70

2.82

376.74

0.591

0.7130

15

−1

1

20

33

385.50

3.00

287.04

1.340

0.8022

16

0

0

25

28

342.16

1.72

223.45

0.635

0.6619

17

1

0

30

28

442.79

0.87

314.91

0.391

0.6600