Figure 5
Response of biosensors 8–9 to increasing concentrations of β-estradiol. (A) Comparison of the performance of biosensor 7 (byMM381) and biosensors 8 (byMM1396, byMM1397). The three of them were built by adding one lex2Op to a different biosensor 6 configuration. (B) Comparative response of biosensor 7 and four variants. The biosensors differ from each other only for the distance between two adjacent lex2Op (2, 6, 9, 15, and 21 nt). In particular, four configurations (2, 6, 15, and 21 nt) appear highly effective and almost undistinguishable from each other. Details about the statistical analysis are given in the main text. (C) Biosensors 9. byMM369 is completely outperformed by byMM381, whereas byMM367 share, with byMM381, the maximal fluorescence value (ns: p-value = 0.5360; two-tailed Welch’s t-test) and has a much lower basal fluorescence. The circuit in byMM367 is not optimal as a biosensor but it is extremely valuable to activate and enhance gene expression at 2000 nM β-estradiol, with basically no leakage when the chemical is absent.
