Figure 3
From: Overexpression of the NEK9–EG5 axis is a novel metastatic marker in pathologic stage T3 colon cancer
Concordant expression of the NEK9–G5 axis during the G2/M phase of the cell cycle. (a) Cell cycle distribution of SW480 and SW620 colon cancer cells treated with nocodazole (500 nM) for 24 or 48 h and then released from this block. (b) Western blot analysis of phosphorylated NKE9 (pNEK9T210), NEK9, EG5, acetyl-tubulin, phosphorylated AKT (pAKTS473) and cyclin B1 at the indicated times of nocodazole–induced G2/M arrest, and after the release from this block.
