Figure 4
From: Low-energy shock wave therapy ameliorates ischemic-induced overactive bladder in a rat model
(A) Gene expression at the early phase (24 h after the first LESW) by real-time PCR (ratio to β-actin). Significant upregulations of VEGF and CD31 were observed in the AI-SW group than in the AI group in the early phase. There was no significant difference among three groups in sGCα1 or sGCβ1. (B) Gene expression at the late phase (24 weeks of age) by real-time PCR (ratio to β-actin). sGCα1 expressions were significantly higher in the control and AI-SW groups than in the AI group in the late phase. There was also higher expression of sGCβ1 in the AI-SW than in the AI group. VEGF expression was higher in the AI group without statistical significance. (C) Protein expression at the late phase by Western blotting (relative expression /β-actin). The expressions of sGCα1 and sGCβ1 were significantly higher in the control and AI-SW groups than in the AI group. VEGF was highly expressed in the control than in the AI group. (D) Cyclic GMP assay. The amount of cGMP (/total protein) was significantly greater in the AI-SW group than in the AI group. *, P < 0.05. All data are presented as mean ± SEM in the graphs.
