Figure 5
From: Low-energy shock wave therapy ameliorates ischemic-induced overactive bladder in a rat model
(A) Hematoxylin and Eosin (HE) staining and (B) Masson’s trichrome staining of the bladder. The suburothelial layer (yellow bars) is thinned in the AI model, while the thinning restored in AI-SW model. (C, D) Immunohistochemistry (IHC) of sGCα1 (C, G4280, Sigma-Aldrich), and sGCβ1 (D, NBP1-89,784, Novus Biologicals). Suburothelial layer is weakly stained in the AI model while a strong deposition can be seen in the AI-SW and control model. Black bars indicate 100 µm. (E) Quantitative analysis of HE staining. Number of hematoxylin-stained nuclei in lamina propria was significantly decreased in the AI group compared to the AI-SW and control groups. (F, G) Quantitative analyses of IHC. The expression of sGCα1 was significantly decreased in the AI group.
