Figure 5
From: Prion protein amino acid sequence influences formation of authentic synthetic PrPSc
Brains of HaHSPWT- or HaHSPΔG54 -infected hamsters are characterized by the histopathological hallmarks of prion disease. Brain sections from mock-infected (UN), second passage HaHSPWT-, and second passage HaHSPΔG54-infected animals were stained with hematoxylin and eosin (a–c) to observe spongiform degeneration. Immunohistochemistry was also performed using the anti-PrP antibody 3F4 (d–f), the astrocyte marker GFAP (g–i), and the microglial marker Iba-1 (j–l) to observe abnormal PrP deposition, astrogliosis, and microgliosis, respectively. The white schematic inset in (A) depicts the brain region imaged in (a–c). The black schematic in (d) depicts the brain region imaged in (d–l). Scale bar 100 μm; inset scale bar 25 μm.
