Table 1 Transmission and adaptation of hamster synthetic prions to hamsters.
From: Prion protein amino acid sequence influences formation of authentic synthetic PrPSc
Inoculum | Incubation period | Clinical duration | Avg. Weight (grams)a | PrPSc Detected by WBb |
|---|---|---|---|---|
Synthetic prions | ||||
Mouse WT | 321 ± 3c (5/5)d | 66 ± 3 | n.d.e | 5/5f |
Hamster WT | \(\ge\) 500 (0/5) | n.a.g | n.a | 5/5 |
Hamster ΔG54 | \(\ge\) 500 (0/5) | n.a | n.a | 5/5 |
Hamster ΔG54/M139I | \(\ge\) 500 (0/5) | n.a | n.a | 0/5 |
Hamster M139I/I205M | \(\ge\) 500 (0/5)h | n.a | n.a | 0/5 |
Uninfected b.h.i | \(\ge\) 500 (0/5) | n.a | n.a | 0/5 |
Brain derived prions | ||||
1st ha. pass. HSPWT b.h. | 335 ± 6 (5/5) | 41 ± 3 | 200 | 5/5 |
1st ha. pass. HSPΔG54 b.h. | 305 ± 5 (5/5) | 60 ± 2 | 196 ± 8j | 5/5 |
Uninfected b.h. | \(\ge\) 400 (0/5) | n.a | 148k | 0/5 |
2nd ha. pass. HSPWT s.c.h.l | 168 ± 3 (5/5) | 57 ± 3 | 198 ± 7 | 5/5 |
2nd ha. pass. HSPΔG54 s.c.h. | 315 ± 10 (5/5) | 81 ± 10 | 214 ± 2 | 5/5 |
Uninfected b.h. | \(\ge\) 430 (0/5) | n.a | 170-175m | 0/5 |
