Figure 1
From: Haploinsufficiency of Shank3 increases the orientation selectivity of V1 neurons
In vivo calcium imaging of L2/3 neurons in V1. (a) Representative image of an AAV.Syn.GCaMP6f.WPRE.SV40 injection into V1. (b) Cartoon of in vivo two-photon calcium imaging in V1 from head-fixed awake mouse and visual stimuli. (c) Representative field of view showing GCaMP6f positive neurons from WT and Shank3+/− mice. Numbers represent examples of somas. Imaging was performed 3–4 weeks after AAV injection, acquired at 5 Hz. (d) Calcium transients (∆F/F0) of the neurons labeled in (c), individual traces (lighter lines), and mean traces (dark lines) are shown from WT (black traces) and Shank3+/− (red traces) mice. Gray background bars indicate the visual stimuli (4 s). (e) Quantifying GCaMP6f positive neurons for both genotypes (Student´s t-test, p = 0.811). Bars represent means ± SEM. (f) Percentage of responsive neurons from individual regions of interest in WT and Shank3+/− mice (Student’s t-test, * p = 0.018). Bars represent means ± SEM.
