Figure 2
From: Haploinsufficiency of Shank3 increases the orientation selectivity of V1 neurons
Neurons from Shank3+/− respond differently to specific stimuli. (a) Color maps showing each responsive neuron activity evoked by visual stimulation for WT (n = 9 mice, 63 responsive neurons) and Shank3+/− mice (n = 9 mice, 105 responsive neurons). Neurons were sorted by their intensity and their preferred stimulus orientation. (b) The mean neuronal activity represented as ∆F/F0 evoked for each stimulus in WT and Shank3+/− mice. Lines indicate the ∆F/F0 mean values, and lighter shadings show the standard error of the mean. Two-way ANOVA revealed that there was a significant interaction between genotype and stimulus (p = 0.0002), followed by Sidak post-hoc test; we found that neurons from Shank3+/− mice showed significant activity differences at 45 degrees (*p = 0.017), and 315 degrees (**p = 0.003). (c) Response amplitude from responsive neurons in WT and Shank3+/− mice (Basal to maximum point) (Mann–Whitney U test, p = 0.318). Represented as median + IQ range. (d) Ramp index from WT and Shank3+/− (Mann–Whitney U test, p = 0.594). Represented as median + IQ range. e) Proportions of responsive neurons for each stimulus. Student’s t-test, * p = 0.046 for 45° and *p = 0.043 for 315°. Bars indicate means ± SEM. (f) Percentage of neurons with preferred orientation from WT and Shank3 ± mice. *p = 0.032, Mann–Whitney two-tailed test. Represented as means ± SEM. (g) Cumulative probability and histogram for preferred orientation distributions, Kolmogorov–Smirnov, p = 0.16.
