Figure 3 | Scientific Reports

Figure 3

From: The interrelationships between neuronal viability, synaptic integrity, microglial responses, and amyloid-beta formation in an in vitro neurotrauma model

Figure 3

Decreased neuronal expressions of APP (A; [F(3,20) = 20.64, P < 0.0001]) and increased expressions of both BACE1 (B; [F(3,20) = 12.32, P < 0.0001]) and Aβ (C; group factor [F(3,40) = 12.8, P < 0.0001], Aβ factor [F(1,40) = 4.301, P = 0.0446], interaction [F(3,40) = 0.6942, P = 0.561]) in the injured-neurons can be reversed by coculture with BV2. In contrast, the neuronal levels of APP in the intact neurons were reduced by coculture with injured-BV2 (D; [F(2,18) = 88.89, P < 0.0001]), whereas the neuronal levels of both BACE1 (E; [F(2,18) = 15.19, P = 0.0001]) and Aβ (F; group factor [F(2,36) = 11.18, P = 0.0002], Aβ factor [F(1,36) = 1.463, P = 0.2343], interaction [F(2,36) = 1.118, P = 0.338]) were enhanced by coculture with injured-BV2. Representative immunoblots are shown. β-actin was used as a loading control. Measurements were made in triplicate, and each bar represents the mean ± SD. One-way ANOVA (for APP and BACE1 expression values) and two-way ANOVA (for Aβ expression values) with Tukey’s test were used for multiple comparisons.

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