Figure 4 | Scientific Reports

Figure 4

From: The interrelationships between neuronal viability, synaptic integrity, microglial responses, and amyloid-beta formation in an in vitro neurotrauma model

Figure 4

When cocultured with intact neurons or injured neurons, BV2 cells maintain their normal phagocytic capacity. The phagocytic capacity of BV2 cells was decreased by cytochalasin D or stretch injury. Representative flow cytometry plots are shown (left panels). Quantification of bead uptake (%) and BV2 cell phagocytic capacity is presented (right panels). Each bar represents the mean ± SD of 6 independent samples per experimental condition. (C) There was a significant difference between the Negative control (cytochalasin-treated BV2, BV2 + Cyto.D) and the BV2 group [F(5,30) = 146.8, P < 0.0001]. (D) The injured BV2 cell and neuron + injured BV2 cell groups exhibited significantly lower phagocytotic capacity than that in the BV2 cell and neuron + BV2 cell groups [F(6,28) = 97.1, P < 0.0001]. One-way ANOVA with Tukey’s test was used for multiple comparisons.

Back to article page