Table 3 Model reaction to assess CYP-activity.
From: Periportal steatosis in mice affects distinct parameters of pericentral drug metabolism
Model reaction | CYP enzymes | CYP-isoforms | Measured metabolite |
|---|---|---|---|
Ethylmorphine-N-demethylation (EMND) | CYP3A54 | 3A1, 3A11, 3A13, 3A16, 3A2, 3A4 | Formaldehyde |
Ethoxycoumarin-O-deethylation (ECOD) | CYP1A, 2A, 2B, 2C55 | 1A2, 2A1, 2A6, 2B1, 2B6, 2C11, 2C9 | 7-Hydroxycoumarin |
Ethoxyresorufin-O-deethylation (EROD) | CYP1A56 | 1A1, 1A2 | Resorufin |
p-Nitrophenol-hydroxylation (PNPH) | CYP2E157 | 2E1 | 4-Nitrocatechol |
Pentoxyresorufin-O-Depentylation (PROD) | CYP2B56 | 2B1, 2B6, | Resorufin |
