Figure 2
From: Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins
Interactome analysis of TESMIN. (a) Schematic overview of sample preparation for mass spectrometry following immunoprecipitation and biotinylated protein pull-down. (b, c) Venn diagram of IP-Mass (b) and proximity labeling proteomics (c) analysis. Two mice were used for each experimental group. (d) Venn diagram of proteins that were reproducibly captured in IP-MS (b) and proximity labeling proteomics (c) using KO, BioID2-Tg mice. Proteins detected at least once in the wild type are excluded (described as specific). (e) The list of specific proteins in d and quantitative values (normalized total spectra). The whole data is available in Dataset S1. (f) Schematic overview of intraperitoneal administration of biotin. (g) Venn diagram of proteins that were reproducibly captured in proximity labeling proteomics using vehicle- and biotin-injected KO, BioID2-Tg mice. Proteins detected at least once in the wild type are excluded (described as specific). (h) The list of specific proteins in g and quantitative values (normalized total spectra). The whole data is available in Dataset S1.
