Figure 3
From: Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins
Immunoprecipitation and immunostaining of representative proteins. (a, b) Immunoblot analysis for MYBL1, LIN9, and DVL2 using eluted samples of IP (a) and proximity labeling proteomics (b). Red arrows indicate the expected protein size. (c) Immunoblot analysis for TESMIN-BioID2-3xFLAG using eluted samples of immunoprecipitation using anti-MYBL1, -LIN9, and -DVL2 antibodies. (d, e) Significance analysis of interactome (SAINT) and log2 fold change of average spectrum count are shown as scatterplots for immunoprecipitation-mass (d) and proximity labeling proteomics (e). The led dots indicate TESMIN. (f) The human orthologs of mouse proteins that were captured in proximity labeling proteomics using Tesmin-BioID2 transgenic mice (Fig. 2e). Human data were referred to because no mouse data are deposited. The found/total column shows the number of proximity labeling experiments that captured each protein in the contaminant repository for affinity purification (CRAPome) database.
