Figure 2

Incomplete rolling of membrane patches in the presence of ANXA4 and ANXA6 mixtures. Response of non-vesicular membrane patches with open edges (DOPC/DOPS, 9:1 molar ratio) to exposure to a mixture of 40 nM ANXA4 with 20 nM (A–E) and 40 nM (F–J) ANXA6. Molecular dynamics simulation of ANXA6 with membrane. (K) Final simulation snapshot. POPC lipids are shown in grey and POPS lipids are in blue. Ca²⁺ ions are shown as green spheres (L) Curvature profile for a surface passing through the center of the membrane with ANXA6 (M) Curvature profile for a surface passing through the center of the membrane without ANXA6. Theoretical modelling of the rolled membrane length L (N,O). Change in the rolled length L as a function of an increase in the adhesion energy w_ad relative to the reference value (N). Change in the rolled length L as a function of a reduction in spontaneous curvature c₀ relative to the reference value (O). Images prepared using NIS elements, version 5.10 (https://www.microscope.healthcare.nikon.com/products/software/nis-elements) and Visual Molecular Dynamics, version 1.9.3 (https://www.ks.uiuc.edu/Research/vmd/).