Figure 3
From: Interplay of membrane crosslinking and curvature induction by annexins
Localization of crosslinkers during membrane patch rolling. Response of non-vesicular membrane patches with open edges (DOPC/DOPS, 9:1 molar ratio) to exposure to annexins when 1:1 annexin mixtures of ANXA4 and ANXA1-GFP (DiD, A–E; GFP, F–J), ANXA4 and ANXA2-GFP (DiD, M–Q; GFP, R–V) and ANXA4 and ANXA6-GFP (DiD, Y–C′; GFP, D′–H′)) are added to non-vesicular membrane patches with open edges (DOPC/DOPS, 9:1 molar ratio). Cross-sections taken on rolling onset (white line ANXA1, B,G, ANXA2, N,S, ANXA6, Z,E′) show that maximum ANXA-GFP fluorescence intensity (green line) coincides with the membrane patch edge as defined by DiD fluorescence (red line) for ANXA1 (K) and ANXA2 (W) and has no maximum for ANXA6 (I′).Cross-sections taken on rollup completion (white line ANXA1, E, ANXA2, Q, ANXA6, C′) show that maximum ANXA-GFP fluorescence intensity (green line) coincides with the rolled membrane as defined by DiD fluorescence (red line) for ANXA1 (L), ANXA2 (X) and ANXA6 (J′). Images prepared using NIS elements, version 5.10 (https://www.microscope.healthcare.nikon.com/products/software/nis-elements).
