Figure 4
From: Endothelial dysfunction in Marfan syndrome mice is restored by resveratrol
Elastin sheet disruptions in MFS mice are restored by resveratrol treatment. (a) Representative images of the elastin lamellae in ascending aorta sections visible after Lawson staining. Scale bar indicates 200 µm. (b) Bar plot comparing the number of elastin fiber breaks in the ascending aorta of 36 week old mice. WT (N = 3; M/F 0/3) n = 9 images, MFS-CTRL (N = 7; M/F 0/7) n = 21 images, and MFS-RESV (N = 7 M/F 0/7) n = 21 images. (c) Image analysis of autofluorescent internal elastic lamina (IEL) captured by en face confocal imaging. Wavy elastin lamellae with fenestrae are shown in maximal projection. Quantification of the disrupted area (in red) is performed and presented as percentage of the total elastin area. (d) Representative images of IEL in 20 weeks old WT and Fbn1C1041G/+ MFS mice in top panel, and of 36 weeks MFS-CTRL and MFS-RESV in the bottom panel. Scale bar indicates 50 µm (e) Bar plot comparing the fraction of disrupted IEL in WT WT (N = 4; M/F 0/4) n = 12 images versus MFS (N = 8; M/F 0/8) n = 25 images (left) and MFS-CTRL (N = 6 M/F 0/6) n = 23 images versus MFS-RESV (N = 6 M/F 0/6) n = 22 images (right). All quantified data represents mean ± SD with indicated data points.
