Table 3 List of primer pairs and genetic diversity information.
Locus name | Primer sequences (5′–3′) | Repeat Motif | Distribution | Product size (bp) | Na | Ho | He | PIC | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
DNA | cDNA | DNA | cDNA | DNA | cDNA | DNA | cDNA | |||||
3MaT | F-TTCTTGATGGCATGGACTTC | (A)13 | 5′-UTR | 291 | 5 | 2 | 0.97 | 0.2 | 0.6 | 0.34 | 0.53 | 0.27 |
R-TTGGAAGTTGAACCAACAGC | ||||||||||||
CHI-1 | F-GCTTGCTGGTAAGTGGAAGG | (T)10 | 5′-UTR | 219 | 5 | 4 | 0.85 | 0.33 | 0.59 | 0.39 | 0.53 | 0.36 |
R-AAGCCGGCAAGTTAGTTGAT | ||||||||||||
CHS-1 | F-GCTTCAGATCTTGATATAGTTGGTG | (T)11 | 5′-UTR | 173 | 4 | 3 | 0.69 | 0.61 | 0.51 | 0.61 | 0.44 | 0.54 |
R-CGAGAATTACTCACTGCCAACA | ||||||||||||
CHS-3 | F-AATGGCGTTGAAGCTGAACT | (A)13 | 5′-UTR | 146 | 4 | 2 | 0.25 | 0.03 | 0.22 | 0.06 | 0.21 | 0.06 |
R-CGGAGGCTACAGTGGTGATT | ||||||||||||
LCYE-1 | F-AACAGTGTCCTCCTCTCCATT | (T)21 | 5′-UTR | 204 | 4 | 8 | 1 | 0.94 | 0.56 | 0.74 | 0.47 | 0.7 |
R-GGTGGCGACTGGTTATCATT | ||||||||||||
PSY-1a | F-AACCAGTGTCCAACACAACAAC | (GT)7 | 5′-UTR | 265 | 5 | 4 | 0.9 | 0.35 | 0.55 | 0.65 | 0.45 | 0.58 |
R-ATGAGCATCACCAGCAGAGC | ||||||||||||
PSY-1b | F-GGTGGCTAATCCAACTGGTG | (AAG)5 | CDS | 160 | 5 | 5 | 0.65 | 0 | 0.5 | 0.59 | 0.46 | 0.54 |
R-GTTCCAGGAAGCACAATATCC | ||||||||||||
LCYE-4 | F-GCATCATAGGAGGTGTGATGG | (GAG)5 | 5′-UTR | 185 | 4 | 4 | 0.18 | 0 | 0.17 | 0.54 | 0.16 | 0.49 |
R-CGGACAATCTGGACTGAGAA | ||||||||||||
Average | 4.5 | 4 | 0.69 | 0.31 | 0.46 | 0.49 | 0.41 | 0.44 | ||||
