Figure 4 | Scientific Reports

Figure 4

From: A novel approach for the endothelialization of xenogeneic decellularized vascular tissues by human cells utilizing surface modification and dynamic culture

Figure 4

Recellularization of decellularized tissues using a vessel container. (a) A custom-made bioreactor system for dynamic cell culture using a vessel container. Upper: Entire system. (i) Console, (ii) Rotor, (iii) Plate type container (as shown in Fig. 1a), (iv) Vessel container, (v) Culture medium reservoir. Lower: Vessel container. Decellularized tissue is installed inside. (b) Flow simulation. Upper: Vector. Lower: Streamline. The vector image indicates the flow direction and travel in a tube container with arrow marks. The streamline image indicates the flow speed with colors. (c) Representative fluorescence images after static (upper) and dynamic (lower) culture. The results of three independent experiments are shown. Higher magnification images for #3 is also provided (right). Scale bars: 2 cm in (a) (lower), 5 mm in (c) (main) and 1 mm in (c) (right).

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