Figure 6
Protein sequence alignment of AmiA from S. pneumoniae strains D39, R6 and ATCC 17619; clinical S. pseudopneumoniae isolate (410.05) and clinical S. mitis isolate (3308.46). Amino acids highlighted in grey are part of the N-terminal signal peptide. Amino acids highlighted in red were predicted to be at the AmiA peptide ligand-AmiA protein interaction interface by PepNN, interaction propensity/probability of ≥ 0.5. Identical residues are represented by a dot(.). Translational stop codon represented by an underscore (_). Note that the only sequence difference between D39 and S. pseudopneumoniae (inhibited by AmiA peptide ligand) and R6 and S. mitis (not inhibited by AmiA peptide ligand) is at position 54 (indicated by blue arrow), predicted to be within the binding site.
