Table 4 Prediction of p.Ser807Arg mutatant protein by using Mupro, I-Mutant 2.0(Seq), iStable and HOPE.
From: Mutation analysis of the WFS1 gene in a Chinese family with autosomal-dominant non-syndrome deafness
| Â | Domain | Â | Consurf score | Â | Conservation | Â |
|---|---|---|---|---|---|---|
Evolutionary conservation | C-terminal | Â | 9 | Â | Neither the mutant residue nor another residue type with similar properties was observed at this position in other homologous sequences. Based on conservation scores this mutation is probably damaging to the protein | Â |
| Â | MUpro | I-Mutant 2.0(Seq) | iStable | |||
|---|---|---|---|---|---|---|
Confidence score | Prediction | DDG | Prediction | Confidence score | Prediction | |
Stability | 0.31181111 | Increase | − 0.1 | Increase | 0.843898 | Increase |
| Â | Change of size | Â | Change of charge | Â | Change of Hydrophobicity | Â |
|---|---|---|---|---|---|---|
HOPE | Mutant type>wild type |  | Neutral→positive |  | Decrease |  |
The mutant residue is bigger, this might lead to bumps. | Â | The mutation introduces a charge, this can cause repulsion of ligands or other residues with the same charge. | Â | Hydrophobic interactions, either in the core of the protein or on the surface, will be lost. | Â |
