Figure 2
From: Anti-biofilm effects and healing promotion by silver oxynitrate-based dressings
Visualisation of P. aeruginosa biofilms after treatment with silver dressings. (A–D) The viability of bacteria within P. aeruginosa biofilms grown on porcine skin were visualised with PrestoBlue viability dye after 24 h treatment with silver dressings or non-antimicrobial control dressings. Viable bacteria appear pink and non-viable bacteria and porcine skin appear blue. (E) Viable bacteria (pink staining) were quantified using Image Pro version 10 (F–I) Scanning electron microscopy of P. aeruginosa biofilms grown on porcine skin and treated with silver dressings or non-antimicrobial control dressing for 24 h. SEM scale bar = 5 µm. (J–M) Colony biofilms grown on filter membranes and stained with PrestoBlue viability dye following 24 h incubation with silver dressings.
